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IU Bloomington team plays role in Human Microbiome Project

June 21, 2012

In mid-June, the news media were abuzz with stories about the Human Microbiome Project, an ambitious project spearheaded by the U.S. government’s National Institutes of Health to map the normal microbial make-up of healthy humans. Among the 200 researchers at nearly 80 universities and institutions contributing to the project’s release of new data was a team of researchers led by IU Bloomington assistant professor of informatics and computing Yuzhen Ye.

Ye’s team of researchers conducted an in-depth and diverse genetic analysis of the defense systems that trillions of micro-organisms in the human body use to fend off viruses. Their work is among a collection of 16 research papers released by the Human Microbiome Project Consortium. The new data is the result of five years of work and an investment of $173 million.

The IU team of bioinformaticists and biologists reconstructed arrays of clusters of regularly interspaced short palindromic repeats — CRISPRs — which function as immune systems to bacteria that play a vital role in human health. Between genomic repeats, CRISPR locations carry short strands of foreign DNA called spacers, which provide a history of past exposures to outside invaders like plasmids and bacteriophages (viruses that infect bacteria) and allow the bacteria to fight off viruses they have already encountered.

“By studying CRISPRs and their sequences, we ask: Do individuals make antibodies to a particular virus? If they do, we then know they have been exposed to that virus,” Ye says. “By examining CRISPR sequences, we learn about what viruses there have been infecting different species of bacteria in a particular environment.”

Bacteriophages are the most abundant life form on the planet and are in a constant arms race with bacteria, which in the human body outnumber human cells by 10 to 1. Scientists want to better understand how microbes — a group that contributes more genes responsible for human survival than humans themselves do — battle the viruses that seek to infect them.

The Human Microbiome Project collected tissues from 15 body sites in 129 men and from 18 body sites in 113 females, with up to three samples taken from each volunteer’s mouth, nose, skin, and lower intestine, in addition to three vaginal sites in women. The IU team confirmed that by using targeted assembly, longer CRISPR arrays were produced that allowed more spacers to be identified for analyzing CRISPR evolution. For 142 out of 150 CRISPRs, their traces were identified in more datasets by targeted assembly as compared to whole metagenome assembly, and for 36 CRISPRs, they were seen in at least 10 times more datasets.

“We know that CRISPRs adapted to a virus or other infectious agent are extremely important to the bacteria carrying those CRISPRs: They live or die,” Ye says. “But we really don’t understand how this leads to changes in the entire biology of an individual.”

The team found a skin-specific CRISPR (PacneL29) in Propionibacterium acnes — the only skin-specific CRISPR found in the Human Microbiome Project datasets — and P. acnes is linked to the skin condition acne, which is one of the many cases where a complicated interaction between host and bacteria exist. While most people have the bacteria, they don’t have acne.

“Overall, this work demonstrates the applications of CRISPRs to tracing the virus exposure of individuals,” Ye says. “And it indicates the importance of effective identification and characterization of CRISPR loci to the study of the dynamic ecology of microbiomes.”

The lack of common spacers between individuals probably reflects different individual virus exposure and indicates that the battle between bacteria and viruses is constant, with bacteria adapting to viruses and viruses finding new ways to avoid CRISPR defenses.

“One constant message of the Human Microbiome Project is that people are each unique in the balance of bacteria inhabiting their bodies. CRISPR arrays can be a unique characteristic of an individual at a certain time, so we may be able to use spacer changes as historical markers,” Ye says. “We might also be able to use CRISPR loci as a new molecular fingerprint to identify and trace human individuals from human residues for forensic purposes.”

More generally, scientists want to use CRISPRs to follow the ecology of the human microbiome, and Ye can’t wait for different datasets to study — particularly datasets from samples taken from the same individual over months and years. “One could argue that scientists often learn the most from long-term observations that follow changes at particular sites,” she says.

Diverse CRISPRs evolving in human microbiomes” was published today in PLoS Genetics. Co-authors with Ye were postdoctoral researcher Mina Rho and doctoral student Yu-Wei Wu, both of the School of Informatics and Computing, associate professor Haixu Tang of the School of Informatics and Computing and director of bioinformatics at IU’s Center for Genomics and Bioinformatics, and postdoctoral research associate Thomas G. Doak of the IU Bloomington College of Arts and Sciences’ Department of Biology.

The Public Library of Science (PLoS) has provided an open access collection citation and links to each of the 14 Human Microbiome Project research papers available through that publisher.

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